Laboratory biosafety manual third edition is a wellwritten very well organized volume that provides the reader with a clear understanding of biosafety in the laboratory. Tuberculosis laboratory biosafety manual ncbi bookshelf. Biosafety in the laboratory access to the laboratory is controlled and restricted to habilitated staff only. Canadian biosafety handbook, second edition canada. Laboratory staff, from the laboratory directors on down, need to take responsibility for the biosafety within their facilities and ensure that staff are properly trained and that their facilities meet present containment requirements. Who extended biosafety advisory group bag meetings, geneva, 2425 november 2014, and 15 december 2016 key recommendation 2014 revision to the who biosafety manual is both a necessary and a priority key recommendation 2016 general agreement for the proposed modifications to the manual, the revision of which remains a priority. Its goal is to help prevent labacquired infections while allowing researchers to work with organisms of various levels of hazard. Cohen, centers for disease control and prevention, atlanta, ga, usa. The risk of laboratoryacquired viral infection is illustrated by a number of case reports.
Biosafety in microbiological and biomedical laboratories. Who laboratory biosafety manual having served for global biosafety community hereinafter, who manual for more than 20 30 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Laboratory biosafety for handling emerging viruses. Chapman university is using the bmbl fifth edition, 2009 as the basis for this biosafety manual. Biosafety manual california institute of technology. National lab biosafety and biosecurity task force to develop nlbbp administrative policy, manual of standards and guidelines task working group members of lab biosafety task force of the dept.
This biosafety manual has been developed by the environmental health and safety ehs department at michigan state university. Vacuum lines are protected with liquid disinfectant traps. Activities that are specifically addressed are those involving. Laboratory biosafety manual third edition english, world health organization, 2014. If you would like to receive updates as the work on the biosafety manual progresses. Laboratory biosafety manual world health organisation. The unit transformed into the national biosafety management agency on the actualization of the national biosafety management agency act 2015. Baseline serum samples for all laboratory and other atrisk personnel should be collected and stored.
Preparing a laboratory biosafety manual ehs sop biosafety training. Review of laboratory biosafety manuals and standard operating procedures sops for compliance with. Biosafety level refers to the stringency of biocontainment precautions deemed necessary by the centers for disease control and prevention cdc for laboratory work with infectious materials. The maximum containment laboratory biosafety level 4 6. Presentation at the 20th nmfp meeting held on 45 june 2019 at ecdc in stockholm, sweden. The dose, or number of organisms required to cause disease can vary widely depending of the specific organism and the route of entry. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in. Two cases of laboratoryacquired west nile virus infections were recorded in the usa in 2002 through percutaneous inoculation. In particular for biosafety level 4 bsl4 investigation, guidelines for biosafety and biosecurity. Usman took over the headship of the special biosafety unit. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. California occupational safety and health administration calosha. The goals of the biological safety program, referenced in this manual as the biosafety program, are to protect laboratory workers, the public, and the environment from potentially hazardous biological agents. Sample laboratory biosafety manual for microbiological.
Emmett barkley, howard hughes medical institute, chevy chase, md, usa dr murray l. The practices and equipment prescribed for each level are specific for the organisms used, the operations performed, the routes of transmission of the infectious agents used, and the laboratory. Illumination is adequate for all activities, avoiding reflections and glare that could affect vision. Good microbiological technique and appropriate use of biosafety equipment by welltrained staff remain the fundamental elements of laboratory biosafety. Chapman university biosafety manual 3 while not a regulation, the bmbl has been revised several times and is commonly seen as the gold standard for biosafety. Guidelines for laboratoryfacility certification part ii. Laboratory biosafety guidelines, 3rd edition, 2004 phac. Developing a culture of biosafety laboratory practices. The levels of containment range from the lowest biosafety level 1 bsl1 to the highest at level 4 bsl4. The development of this third edition of the laboratory biosafety manual has been made possible through the contributions of the following, whose expertise is gratefully acknowledged. Rufus ebegba took over headship of the unit on the retirement of mr. The guidelines also serve as a technical document providing information and recommendations on the.
Biological laboratory safety training risk group classification nih guidelines for research involving recombinant dna molecules, 2011 who laboratory biosafety manual, 3rd edition risk group 1 agents that are not associated with disease in healthy adult humans. Developing a culture of biosafety is intended for any laboratory performing testing on infectious agents or clinical specimens that could contain infectious agents in the united states. The ibc advocates the use of biosafety precautions that effectively reduce or. The biosafety manual provides information, guidelines, and policies that should be used in conjunction with other resources to safely work with and in the. Who laboratory manual for the examination of human semen and spermcervical mucus interaction. It is designed to provide laboratories with the broad recommendations for. Pdf biosafety training is an effective approach of increasing biosafety and biosecurity capacity of laboratorians working in a. Who laboratory biosafety manual revision update slideshare. Major revisions to the 2014 institutional laboratory. Introduction this manual is intended to be a resource for information, guidelines, policies, and procedures that will enable and encourage those working in the laboratory environment to work safely and reduce or eliminate the potential for exposure to biological hazards. The biosafety manual also intends to aid in the protection of laboratory personnel and the surrounding environment from possible exposure to biohazardous material. The manual now covers risk assessment and safe use of recombinant dna technology, and provides guidelines for the comissioning and.
Laboratory biosafety levels unc gillings school of. The princeton university biosafety manual is intended to be a resource for information, guidelines, policies, and procedures that will enable and encourage those working in the laboratory environment to work safely and reduce or eliminate the potential for exposure to. In tajikistan, the brm training supported a european union and. Laboratory biosafety and biosecurity risk assessment.
Laboratory facilities are designated as basic biosafety level 1, basic biosafety level 2, containment biosafety level 3, and maximum containment biosafety level 4. As this manual does not address the chemical and physical hazards commonly encountered in the laboratory, it is to. Laboratory biosecurity concepts are introduced, and the latest regulations for the transport of infectious substances are reflected. Who laboratory manual for the examination and processing. The most important factor in the likelihood of a laboratoryacquired infection is the pathogenicity of the organism being handled. A manual of written standard operating procedures sops for the laboratory, in combination with this manual and the cdcnih publications biosafety in microbiological and biomedical laboratories, 5th edition and guidelines for research involving recombinant or synthetic nucleic acid molecules, provide general requirements for. The biosafety manual is designed in accordance with nih guidelines for research involving recombinant or synthetic nucleic acid molecules and the biosafety in microbiological and biomedical laboratories bmbl, 5th edition to assure laboratory personnel that with proper precautions, equipment, and facilities most biohazardous materials can be handled without. This manual would be an excellent addition to the laboratory library. The third edition of the who laboratory biosafety manual is a helpful reference and guide to nations that accept the challenge to develop and establish national codes of practice for securing microbiological assets, yet ensuring their availability for clinical, research and epidemiological purposes.
Biosafety manual office of environmental health and safety. A laboratoryacquired infection is known or suspected, or b. Additional serum specimens may be collected periodically, depending on the agents handled or the function of the laboratory. Who laboratory biosafety manual lbm revision update. For more than 20 years, since it was first published in 1983 the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Manual, the us biosafety in microbiological and biomedical laboratories, and the international consensus document cen workshop agreement cwa 15793. At ryerson university, safety means that lab personnel are protected from exposures to infectious agents. This biosafety manual represents the institutional practices and procedures for the safe use and handling of biological materials, recombinant dna and synthetic nucleic acids at stanford university. Major revisions to the 2014 institutional laboratory biosafety manual. Section iv laboratory biosafety level criteria, page 59. No or low individual and community risk a microorganism that is unlikely to cause.
Mycobacteriology laboratory manual v list of abbreviations and acronyms afb acid fast bacilli ast antimycobacterial susceptibility testing. The laboratory biosafety guidelines were initially developed to guide government, industry, university, hospital, and other public health and microbiological laboratories in their development of biosafety policies and programs. This biosafety manual is applicable to all laboratory, research, teaching, service, and support activities that may involveexposure to biohazard agents or materials ous or otherwisecomes under the purview of the institutional biosafety committee ibc at the university of montana. Biosafety is the section of environmental safety that addresses working safely in the laboratory with microorganisms and rdna organisms whose genetic material has been altered. The primary purpose of the biosafety manual is to provide an appropriate training and reference tool relative to biosafety for laboratory staff, as discussed in the ehs sop, biosafety training. A spill of any quantity involving an agent infectious to humans, plants, or animals occurs in a public area. Who laboratory manual for the examination and processing of human semen 5th ed. Whoeurope organized brm training in tajikistan 20 and turkmenistan 2014.
In the united states, the centers for disease control and prevention cdc have specified these. The laboratory biosafety manual should be a living, working document that is an important resource for personnel engaged in the activities using biological and or rdna materials. Receive training in standard microbiological techniques. Biosafety level designations are based on a composite of the design features, construction, containment. With respect to the work with emerging viruses, the laboratoryacquired infection is a reality that cannot be ignored.
The manual is part of msus biosafety program, which was established to accomplish the following goals. Medical laboratory science program biosafetysafety. Who 2004, laboratory biosafety manual 3rd ed, world. Laboratory biosafety manual world health organization. The medical laboratory science program biosafetysafety manual has been prepared for the benefit of those who handle or work in proximity to potentially infectious biological agents. Laboratory biosafety is the process of applying a combination of administrative controls, containment principles, practices and procedures, safety equipment, emergency preparedness, and facilities to enable laboratory staff to work safely with potentially infectious microorganisms. Biosafety level bsl and animal biosafety level absl laboratories for research and diagnostic work using developed laboratory inspection checklists. As needed, an adhoc committee may be formed to consider policy implications for the university of emerging technologies and processes that have a biosafety component. Biosafety manual approved july 28, 2014 page 1 of 17. For this new edition, the manual has been extensively revised and expanded.